Southern blot

Further Southern blot indicated that T-DNA insert was a single copy .

southern杂交结果表明，其T-DNA为单拷贝插入，因此推断该插入功能失活突变体株系的上代单株为杂合体，且为一个插入突变位点。

To investigate the effect of gene amplification and abnormal transcription on expression of cyclin D1 protein . Methods : Protein , mRNA and DNA of cyclin D1 were detected by immunohistochemistry , RT PCR and southern blot , respectively .

方法：免疫组织化学检测cyClinD1蛋白表达，RTPCR检测cyClinD1mRNA的转录异常，southern杂交检测cyClinD1的基因改变。

A Simple , Rapid Method of Southern Blot Analysis

一种简捷的Southern印迹杂交方法

Genomic southern blot analysis suggested the existence of two genes at least .

Southern杂交分析显示，在基因组中至少有两个基因存在。

METHODS Use of southern blot hybridization to detect EGFR amplification .

方法采用Southern印迹法观察脑肿瘤中EGFR的扩增。

The regenerated plants were identified by PCR and Southern blot analysis .

对转基因苗进行分子生物学检测，包括PCR检测和Southem杂交。

Methods PCR amplification and southern blot analysis .

方法PCR反应及Southern杂交分析。

Southern Blot Analysis of Exogenous Transgenic Genes in Transgenic Arabidopsis Plants

转基因拟南芥中外源基因的Southernblot检测

Analysis by PCR and southern blot , seven of them are transgenic .

通过PCR技术和Southern-blot鉴定，获得了七个转基因小鼠系。

Southern blot analysis was used to determine the exogenous gene integration of PCR positive plants .

为进一步确定外源基因的整合情况，对PCR阳性植株进行Southernblot分析。

Study on Homology Between Two Kinds of Human Thymidine Kinase Gene By Southern Blot Analysis

用DNA分子杂交研究两种人体胸苷激酶基因间的同源性

PCR , Southern blot analyses confirmed the transgenic status of some plants .

经PCR、Southernblot分析确认了其中4株独立的转基因植株。

Positive signal fragments were gained by Southern blot hybridization .

进行Southern杂交分析，获得阳性信号条带；

Transgenic mice were identified by PCR and Southern blot ;

PCR及Southernblot鉴定转基因小鼠基因型；

Methods : DNase ⅰ sensitivity assay , Southern blot .

方法：DNaseⅠ敏感性分析法。

Southern blot analysis results show that the exogenous genes are single-copy transgenic plants .

Southern杂交鉴定结果表明，转基因植株的外源基因均为单拷贝。

Southern blot analysis showed that AMS gene is at least three gene copies per genome .

Southernblot分析表明AMS基因在青蒿基因组中至少有3个拷贝。

Southern blot analysis showed that there were two copies of the gene in the three accessions .

Southernblot分析结果表明，三种材料中该基因均含有两个拷贝。

No evidence of processed pseudogenes is found using long PCR and Southern blot .

长片段PCR和Southernblot实验没有发现CYP51假基因。

Technological Improvement of Southern Blot Using Digoxigenin-labeled Probes with Random-primed Method

地高辛随机引物法标记探针的Southern杂交技术优化

Qualitative Polymerase Chain Reaction ( PCR ) and Southern blot are widely used for screening .

定性PCR和Southern印迹是筛选的常用方法。

Southern blot analysis revealed a small FPS gene family in E.pekinensis .

Southern杂交分析结果表明药用植物大戟叶基因组中有一个FPS基因家族。

SCAR marker linked to seedless genes in grapes and southern blot analysis

葡萄无核基因的SCAR标记及Southernblot分析

Prenatal Diagnosis of α - Thalassemia by PCR and Southern Blot

应用PCR和斑点杂交产前诊断α&地中海贫血

Southern blot analysis showed the alien genes integrated in most transgenic plants just by single copy .

Southernblot检测结果同时表明，外源基因在多数转基因植株中表现为单拷贝整合。

The amplification products in the supernatant can be investigated by Southern blot and other analysis .

反应液内的扩增产物还可作Southern杂交及基因分析；

The integration mode in several seedlings from one clone was the same , according to the southern blot analysis .

对来源于同一克隆的几棵转基因植株的Southern分析表明，其整合方式完全一致。

Cotton genomic DNA southern blot analysis indicated that CBF had 3-5 family members in cotton .

通过Southern杂交确定棉花中有3～5个CBF的家庭成员，针对棉花BAC库进行了其他棉花CBF基因的筛选。

Method Southern blot hybridization was used to analyze genomic DNA in liver cancer and its corresponding liver tissue .

方法应用Southern印迹杂交技术，对25例肝癌及癌旁肝组织DNA进行分析。

Southern blot analysis confirmed the presence of gfp recombinant Epstein-Barr virus genomes .

经Southernblot检测证实gfpB958可产生重组gfp的EB病毒。